METHOD FOR CONDITIONING AND CRYOPRESERVING CELLS

摘要

Disclosed is a method for preconditioning and cryopreservation of cells harvested from a donor. Cells are suspended in a cell conditioning and cryopreservation medium and the suspension incubated for a period of at least ten minutes, where the cell medium includes adenosine, a calcium channel blocker, and a cell nutrient matrix comprising a sufficient amount of cell metabolites to sustain the metabolic needs of the cells during incubation without producing detectable levels of lactate or substantially depleting the metabolic substrates of the harvested cells. In some embodiments, a cryopreservative is added in a step-wise before the cell suspension is frozen and removed in a step-wise fashion after the cell suspension is thawed.