| 摘要 |
In the present invention there is disclosed a process of extracting periplasmatic protein and comprising (i) cultivation of prokaryotic micro-organism being transformed by means of expression vector containing a gene encoding said protein and means for expression thereof in periplasm of said micro-organism, (ii) centrifugation or micro-filtration of the obtained cultivation mixture in order to obtain a cellular fraction, optionally lysis of cells of the obtained cellular fraction and centrifugation of the obtained lysate in order to obtain a disintegrated cellular fraction, (iii) suspending the obtained cellular fraction or the disintegrated cellular fraction in a buffer solution, (iv) centrifugation of the obtained suspension in order to obtain a supernatant and (v) isolation of said protein from this supernatant. The invented process is characterized in that the buffer solution contains arginine.
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