发明名称 |
Enzyme-antibody complex and a method for manufacturing the same |
摘要 |
<p>The present invention relates to an enzyme-antibody complex where one or more molecule(s) of enzyme into which a maleimide group or a thiol group is introduced is/are covalently conjugated to a carrier (polylysine, aminodextyan and so forth) in which a thiol group when a maleimide group is introduced into the enzyme or to a maleimide group when a thiol group is introduced into the enzyme via them, and a maleimide group is introduced into at least one amino group remaining in the above complex and is covalently conjugated to a maleimide group of the above complex via a thiol group obtained by reduction of antibody fragment or antibody. The resulting complex is useful as an enzyme-labeled antibody particularly in immunohistochemistry and in enzyme immunoassay and makes an immunoassay with high sensitivity possible.</p> |
申请公布号 |
EP0992794(A2) |
申请公布日期 |
2000.04.12 |
申请号 |
EP19990105375 |
申请日期 |
1999.03.16 |
申请人 |
NICHIREI CORPORATION |
发明人 |
OHABAYASHI, HIROKAZU;KITANO, YURIKO;KITOH, TAKASHI |
分类号 |
G01N33/535;G01N33/543;(IPC1-7):G01N33/535;G01N33/544;G01N33/548;G01N33/563;C12Q1/42;C12Q1/54 |
主分类号 |
G01N33/535 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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