MONOCLONAL ANTIBODY AGAINST HUMAN STROMAL COLLAGENASE OR HUMAN 92 kDa GELATINASE, AND ITS USE

摘要

PROBLEM TO BE SOLVED: To obtain a new monoclonal antibody which is immunoreactive only with an antigen determinant which exists in human stromal collagenase or an antigen determinant which exists in human 92 kDa gelatinase, and is useful for immunohistological analysis of these enzymes and so on. SOLUTION: This is a new monoclonal antibody which is immunoreactive only with an antigen determinant which exists in human stromal collagenase or an antigen determinant which exists in human 92 kDa gelatinase, reacts specifically with potential or active human stromal collagenase or human 92 kDa gelatinase, and is useful for immunohistochemically detecting these enzymes in tissues and cells, and for diagnosing rheumatoid arthritis, cancer, and so on. The monoclonal antibody is obtained by culturing a hybridoma obtained by fusing a mouse spleen cell immunized with these enzymes with a myeloma cell.