发明名称 |
Method for identifying genes encoding signal sequences |
摘要 |
The invention involves a method of identifying nucleic acid sequences encoding signal peptide-containing proteins. The method features chimeric constructs containing a KRE9 gene that lacks a signal sequence. Yeast containing chimeric KRE9 plasmid constructs that encode signal sequences are selected based on their ability to grow on media in which sucrose is the sole carbon source.
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申请公布号 |
US6046000(A) |
申请公布日期 |
2000.04.04 |
申请号 |
US19970966269 |
申请日期 |
1997.11.07 |
申请人 |
MILLENNIUM BIOTHERAPEUTICS, INC. |
发明人 |
MCCARTHY, SEAN A.;KURANDA, MICHAEL JOSEPH;BULAWA, CHRISTINE ELLEN;BOSSONE, STEVEN |
分类号 |
C12N1/19;C12N15/10;C12N15/81;C12Q1/68;(IPC1-7):C12Q1/68;C12P21/00;C12N1/00;C07H21/04 |
主分类号 |
C12N1/19 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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