| 摘要 |
PROBLEM TO BE SOLVED: To efficiently obtain Japanese encephalitis viruses or their antigen components useful for producing vaccines, etc., in a high yield by infecting established cells with the Japanese encephalitis viruses, culturing the infected cells and purifying the viruses or their antigen components from the culture product. SOLUTION: This method for preparing Japanese encephalitis viruses or their antigen components comprises infecting established cells, such as animal or insect-originated cells, selected from the group consisting of Vero cells, GL37 cells (FERM P-16857), HmLu-1 cells, BHK-21 cells, MDCK cells and C6/36 cells with Japanese encephalitis viruses such as Beijin-1 strain or Nakayama strain, culturing the virus-injected cells by a static culture method, a roller bottle culture method, a suspension culture method, etc., and subsequently purifying the viruses or their antigen components from the culture product by a purification method such as a concentration method using an ultrafiltration membrane or an ion exchange or adsorption chromatography. The obtained Japanese encephalitis viruses or their antigenic components are preferably used to prepare Japanese encephalitis virus vaccines.
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