Genetic method for the expression of polyproteins in plants

摘要

A method of expressing or improving expression levels of one or more proteins in a transgenic plant comprising inserting into the genome of said plant a DNA sequence comprising a promoter region operably linked to two or more protein encoding regions and a 3'-terminator region wherein said protein encoding regions are separated from each other by a DNA sequence coding for a linker propeptide, said propeptide providing a cleavage site whereby the expressed polyprotein is post-translationally processed into the component protein molecules. In particular, a signal sequence is also included such that the post-translational processing is effected in the secretory pathway of plants. Suitable linker sequences and DNA constructs for use in the method are also described.