发明名称 |
SYSTEM FOR SCREENING COMPOUNDS WITH ANTITUMORAL, ANTIVIRAL, ANTIPARASITIC AND ANTIFUNGAL ACTIVITY |
摘要 |
Phosphorylcholine (PCho) participates in the transmission of intracellular signals involved in tumoral proliferation. The inhibition of the production of phosphorylcholine (PCho) can be achieved through inhibiting the choline kinase enzyme (Chok). The present invention discloses various alternative and reliable methods for the determination of the activity of the choline kinase ex vivo (in an assay tube, free of cells) or the determination of intracellular levels of Pcho, analysis in vitro (in intact living cells) in the presence or absence of potential compounds having antitumoral activity. The inhibition of the enzyme Chok and the reduction of the levels Pcho would identify said compounds as an antitumoral substance.
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申请公布号 |
WO0012755(A1) |
申请公布日期 |
2000.03.09 |
申请号 |
WO1999ES00275 |
申请日期 |
1999.08.23 |
申请人 |
CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS;LACAL SANJUAN, JUAN CARLOS |
发明人 |
LACAL SANJUAN, JUAN CARLOS |
分类号 |
C12Q1/48;(IPC1-7):C12Q1/48 |
主分类号 |
C12Q1/48 |
代理机构 |
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