发明名称 |
METHODS OF PRODUCING HIGH TITER RECOMBINANT ADENO-ASSOCIATED VIRUS |
摘要 |
The present invention discloses a method of producing large-scale recombinant adeno-associated virus stocks by infection of cells with at least one recombinant adenovirus vector(s). The vector(s) encode a therapeutic gene flanked by the terminal repeat ends of adeno-associated virus and the adeno-associated virus rep and cap genes. Transfection with two recombinant adenovirus vector(s) instead of two plasmids plus adenovirus results in the large scale, high titer production of recombinant adeno-associated virus with little to no contaminating adenovirus present.
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申请公布号 |
WO0011149(A1) |
申请公布日期 |
2000.03.02 |
申请号 |
WO1999US19333 |
申请日期 |
1999.08.24 |
申请人 |
THE UAB RESEARCH FOUNDATION |
发明人 |
MOUNTZ, JOHN, D.;ZHANG, HUANG-GE |
分类号 |
A01N43/04;A01N63/00;C07H19/00;C12N5/00;C12N5/02;C12N7/01;C12N15/00;C12N15/86;C12N15/861;C12N15/864;(IPC1-7):C12N15/00 |
主分类号 |
A01N43/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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