| 摘要 |
<p>An air pressure device for the extraction of DNA and/or RNA througha separation column is disclosed. The device uses a separation column (3) placed onto a waste container (1) via a securing plate (2). After the fluid containing the DNA/RNA is pipetted into the separation column, a plunger (6) is placed into position over the column using the top plate (5) and seal (4). The plunger which is in theup position is pushed down and the resulting increase in air pressure forces the fluid through the barrier in the column which binds the DNA/RNA. The top plate is removed and the same procedure of using the plunger can be used to force the wash solutions through the column barrier. Finally the elution of the DNA/RNA can be done by removing the securing plate (2) with the separation colum (3) and placing the column over collection tubes and using the same procedure to force pre-heated elution solution or water through the barrier, collecting the DNA/RNA in the collection tubes.</p> |
