| 摘要 |
PROBLEM TO BE SOLVED: To simply, accurately measure an elastase originated by granulocyte in a periodontal pocket collecting sample in a short time, by using a periodontal pocket content diluent liquid and granulocytic elastase measuring reagent. SOLUTION: Since white blood cell or particularly granulocyte is early wetted to a gingival inner edge of a periodontal pocket prior to a periodontal change, a diagnosis of a periodontal disease can be rapidly and objectively diagnosed by measuring an elastase originated by the granulocyte. That is, a pediodontal pocket content diluent liquid and granylocytic elastase measuring reagent are used. Gingival groove liquid as a measuring sample, periodontal pocket plaque or the like as a measuring sample is collected by a manual scaler or the like, dissolved, suspended and diluted with diluent liquid. The dilutent liquid is buffer composition and contains, for example, phosphoric acid, tris-hydrochloric acid or the like. As an immulonogical measuring method of the elastase originated by the granulocyte, a particle slide aggregation method, particle turbidimetric method, a solid-phase immunological marker antibody analysis method, an immunochromatography or the like is used.
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