| 摘要 |
<p>A row of (n + m) powder containers which contain powder for chromatography and a row of (n + m) solvent containers which contain solvent for partition are arranged closely in parallel to each other. 0): A predetermined quantity of sample solution is added to the m-th solvent container from the left. 1): The powder containers and the solvent containers of the same numbers from the left in the rows are coupled with each other to form a row of (n + m) pairs of the powder containers and the solvent containers. 2): The powder and solvent of each pair are mixed and stirred until components to be separated come into partition equilibrium. 3): The powder and solvent of each pair are separated and made to exist in the original containers of each pair. 4): The pairs are decoupled to form the row of the powder containers and the row of the solvent containers again, and the rows are shifted from each other by one container in the opposite directions alternately. The steps 1) - 4) are repeated, completing one process composed of the steps 0) - 4) + steps 1) - 4). The process is repeated. Each time the rows are shifted, the leading container containing separated sample component is removed, and a new container is added to the rear of the row to keep the number of containers in the row constant. By this method, the samples can be separated by relatively small number of partition equilibrium steps. <IMAGE></p> |
