发明名称 |
PROCESS FOR PRODUCING CDNA |
摘要 |
The process includes the steps of: a) contacting a liquid containing mRNA with an insoluble support having DNA probes attached thereto via the 5'-terminus thereof whereby the mRNA is hybridised to said probes and hence to said support; b) removing said liquid; and c) adding enzymes and nucleotides in solution whereby the probe functions as a primer to produce single stranded cDNA on the mRNA templates. The DNA probe is oligo-dT or a specific DNA seguence which may correspond to a conserved region in a class of mRNA molecules. The insoluble support may consist of magnet.ic particles which are monodisperse and/or superparamagnetic.
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申请公布号 |
CA2003500(C) |
申请公布日期 |
2000.01.18 |
申请号 |
CA19892003500 |
申请日期 |
1989.11.21 |
申请人 |
HORNES, ERIK;KORSNES, LARS |
发明人 |
HORNES, ERIK;KORSNES, LARS |
分类号 |
C12N15/09;C07H21/04;C12N15/10;C12Q1/68;(IPC1-7):C12N15/10;C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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