| 摘要 |
PROBLEM TO BE SOLVED: To obtain a promotor which consists of DNA which has human MP52 gene promotor domain having a specific base sequence, and is useful, for example, for screening of compounds which have bone-forming activity at a level similar to human BMP protein or inhibiting activity against it. SOLUTION: This promotor is new DNA which has a human MP52 gene promotor domain which has a base sequence of no. 1-3251 in the base sequence shown by the formula, or fragments of it, and is useful, for example, for screening of low molecular compounds which control, positively or negatively, expression of human MP52 using, as an index, a reporter activity of an animal cell in which a recombinant expression vector which is linked to this DNA and an appropriate reporter gene were introduced. Obtained low molecular compounds are useful, for example, for the treatment, for example, of skeletal diseases, chondropathy, tendon lesion, ligament lesion, and neurological disorders, and as anticancer agents. This promotor is obtained by screening a human genome library using a probe which was prepared by PCR of human embryo cDNA using degenerate primer(s). |
