STRAIN OF HYBRID CULTURED CELLS OF ANIMAL MUS MUSCULUS L FOR PREPARING MONOCLONAL ANTIBODIES RAISED TO PROTEIN 105 KDA OF EXCRETORY-SECRETORY ANTIGEN OF HUMAN HEPATIC TREMATODE OPISTHORCHIS FELINEUS

摘要

FIELD: molecular biology, immunology. SUBSTANCE: strain of hybrid transplantable cells IMB-649 is obtained by fusion of cells of murine myeloma P3/NS1/1-Ag4.1 with cells of syngenic mice BALB/c immunized with the purified preparation of the total antigen of hepatic trematode Opisthorchis felineus. The strain of hybrid transplantable cells IMB-649 produces monoclonal antibodies IgG2a that interact specifically with protein 105 kDa of excretory-secretory antigen of Opisthorchis felineus in immunobloting assay. Monoclonal antibodies of this invention are used as reagents for the novel test-systems for human hepatic trematode Opisthorchis felineus. EFFECT: strain indicated above, improved method of antibodies preparing. 1 dwg, 1 tbl, 3 ex