SOLUTION-PHASE ELISA

摘要

A method is disclosed for quantitation of an anlyte in a test solution, wherein the analyte has a specific binding affinity for a detector molecule. The method comprises the steps of attaching a target molecule to the detector molecule via a spacer; contacting the detector-spacer-target molecule complex with the test solution containing the analyte to form a binding mixture; contacting the binding mixture with a solid matrix precoated with capture molecule, which can specifically bind to target molecules; removing unbound materials; and measuring bound materials. Also disclosed is a use of the method of the present invention for the early detection of Insulin Dependent Diabetes Mellitus.