| 摘要 |
<p>Disclosed is a non-fluorescent cyanine dye that may be used as an acceptor in fluorescence energy transfer assays involving the detection of binding and/or cleavage events in reactions involving biological molecules, and assay methods utilising such dyes. The non-fluorescent cyanine dye is a compound of formula (I), wherein the linker group Q contains at least one double bond and forms a conjugated system with the rings containing X and Y; groups R?3, R4, R5 and R6¿ are attached to the rings containing X and Y, or optionally, are attached to atoms of the Z?1 and Z2¿ ring structures; Z?1 and Z2¿ each represent a bond or the atoms necessary to complete one or two fused aromatic rings each ring having five or six atoms, selected from carbon atoms and, optionally, no more than two oxygen, nitrogen and sulphur atoms; at least one of groups R?1, R2, R3, R4, R5, R6 and R7¿ is a target bonding group; any remaining groups R?3, R4, R5, R6 and R7¿ groups are independently selected from the group consisting of hydrogen, C¿1?-C4 alkyl, OR?9, COOR9¿, nitro, amino, acylamino, quaternary ammonium, phosphate, sulphonate and sulphate, where R9 is selected from H and C¿1?-C4 alkyl; any remaining R?1 and R2¿ are selected from C¿1?-C10 alkyl which may be unsubstituted or substituted with phenyl, the phenyl being optionally substituted by up to two substituents selected from carboxyl, sulphonate and nitro groups; characterised in that at least one of the groups R?1, R2, R3, R4, R5, R6 and R7¿ comprises a substituent which reduces the fluorescence emission of said dye such that it is essentially non-fluorescent.</p> |
