| 摘要 |
Detecting antibiotic resistance in microorganisms by in situ characterization of a probe hybridizing with an antibiotic resistance associated nucleic acid in a microorganism is new. A method to detect antibiotic resistance in microorganisms comprises the steps: preparing a microorganism containing test sample; contacting the sample with at least one hybridization probe, specific for an antibiotic resistance associated nucleic acid in the microorganism, under conditions specific for hybridization of the probe; evaluating the sample in situ through characterizing the appearance or failure of hybridization. Independent claims are also included for: a reagent kit for typing microorganisms and/or antibiotic resistance in microorganisms through in situ hybridization; and oligonucleotides designated ClaR1, ClaR2, ClaR3, ClaWT, Hyp1-16S-753, 120b, Hyp1-16S-585 or Hyp1-16S-219 or that is at least 10 nucleotides in length and derived from these. |
