METHOD OF DETERMINING A GENOTYPE BY COMPARING THE NUCLEOTIDE SEQUENCE OF MEMBERS OF A GENE FAMILY AND KIT THEREFOR

摘要

PCT No. PCT/US91/07308 Sec. 371 Date Apr. 14, 1993 Sec. 102(e) Date Apr. 14, 1993 PCT Filed Oct. 8, 1991 PCT Pub. No. WO92/08117 PCT Pub. Date May 14, 1993A method is provided for determining a genotype by comparing the nucleotide sequence of members of a gene system which flank the polymorphous sections of a locus or loci of interest. In a general embodiment of the method, the nucleotide sequences chosen for comparison (i) contain one or two sequences that are completely conserved between the members of the gene family, where two or more members are selected from different sources, and (ii) the completely conserved sequences flank strongly conserved sections of genetic material. The completely conserved sequences are typically used to amplify, from different sources, the strongly conserved sections of genetic material. The resulting amplified nucleic acid sequences from the different sources are then compared to establish genotypes.