发明名称 |
Receptor-activated reporter systems |
摘要 |
The invention discloses a chimeric GLUT transporter including a GLUT receptor polypeptide fused to a domain of a detectable heterologous polypeptide and cells expressing such reporter constructs. Cells expressing a detectably-tagged chimeric GLUT transporter are used in methods of screening candidate compounds for their ability to agonize or antagonize an interaction between a ligand and a receptor, e.g., insulin. In addition, a 30 amino acid intracellular retention sequence is disclosed.
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申请公布号 |
US5989893(A) |
申请公布日期 |
1999.11.23 |
申请号 |
US19940287537 |
申请日期 |
1994.08.08 |
申请人 |
UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER |
发明人 |
CZECH, MICHAEL P.;CORVERA, SILVIA |
分类号 |
C07K14/62;C12N1/21;G01N33/50;(IPC1-7):C12N1/21 |
主分类号 |
C07K14/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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