发明名称 |
DNA ligase assay |
摘要 |
A quantitative and functional DNA ligase assay is disclosed. The assay involves the disruption, using restriction enzymes, of a plasmid containing a reporter gene, followed by a ligation step performed in the presence of the biological sample being assayed for DNA ligase activity. The ligation reaction products are then subjected to a coupled transcription-translation reaction, and the extent of DNA ligation is then quantified.
|
申请公布号 |
US5976806(A) |
申请公布日期 |
1999.11.02 |
申请号 |
US19980085902 |
申请日期 |
1998.05.27 |
申请人 |
PIONEER HI-BRED INTERNATIONAL, INC. |
发明人 |
MAHAJAN, PRAMOD B.;BOWEN, BENJAMIN;TAGLIANI, LAURA A. |
分类号 |
C12Q1/25;C12Q1/68;(IPC1-7):C12Q1/68;C12Q1/00;C12Q1/66;C12P19/34 |
主分类号 |
C12Q1/25 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|