发明名称 |
Method of preparing a variant of a lipolytic enzyme |
摘要 |
The present invention relates to a method of preparing a variant of a parent lipolytic enzyme, comprising (a) subjecting a DNA sequence encoding the parent lipolytic enzyme to random mutagenesis, (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and (c) screening for host cells expressing a mutated lipolytic enzyme which has a decreased dependance to calcium and/or an improved tolerance towards a detergent or a detergent component as compared to the parent lipolytic enzyme.
|
申请公布号 |
US5976855(A) |
申请公布日期 |
1999.11.02 |
申请号 |
US19960701339 |
申请日期 |
1996.08.22 |
申请人 |
NOVO NORDISK A/S |
发明人 |
SVENDSEN, ALLAN;CLAUSEN, IB GROTH;OKKELS, JENS SIGURD;THELLERSEN, MARIANNE |
分类号 |
C12N15/09;C11D3/386;C11D7/42;C12N1/19;C12N1/21;C12N9/20;C12N15/55;C12R1/69;(IPC1-7):C12N9/20;C12N1/20;C12N1/00;C07H21/04 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|