发明名称 |
PROCESS FOR THE RECOVERY AND PURIFICATION OF A RECOMBINANT PROTEIN FROM A CELL |
摘要 |
The present invention involves a process of purifying and recovering a recombinant protein from a suspension of cells. The process of the present invention involves extracting a recombinant protein from a concentrated suspension of cells using a water-miscible organic solvent, isolating the recombinant protein from the suspension of cells, concentrating the recombinant protein to remove the water-miscible organic solvent, precipitating the recombinant protein using an acid, washing the recombinant protein with the salt or free form of a suitable organic acid and recovering the recombinant protein. The method is particularly applicable to the recovery and purification of recombinant beta-casein.
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申请公布号 |
CA2326374(A1) |
申请公布日期 |
1999.10.28 |
申请号 |
CA19992326374 |
申请日期 |
1999.04.15 |
申请人 |
ABBOTT LABORATORIES |
发明人 |
ARNDT, JOSEPH;SCHWARTZ, ELLEN MARIE;LIST, JULIE R.;CARNEY, RONALD E. |
分类号 |
C12N15/09;C07K1/36;C07K14/47;(IPC1-7):C07K14/47 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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