METHOD OF PRODUCING PLANTS WHICH ARE TOLERANT OR RESISTANT TO HERBICIDES

摘要

A method of making plants which are resistant or tolerant to herbicides which, in vitro, inhibit 4-hydroxyphenylpyruvate dioxygenase (4HPPD) comprises the steps of: (i) transforming plant material with a polynucleotide comprising a region encoding a phytoene desaturase; (ii) regenerating the thus transformed material into morphologically normal plants. In a preferred embodiment the region comprised by the polynucleotide is the sequence depicted in SEQ ID No.1, or is a sequence which is complementary to one which when incubated at a temperature of between 55 and 60 DEG C in 0.3 strength citrate buffered saline containing 0.1 % SDS followed by rinsing at the same temperature with 0.3 strength citrate buffered saline containing 0.1 % SDS still hybridises with the sequence depicted in SEQ ID No.1.