Expressionssystem zur Produktion von Proteinen

摘要

The invention relates to an expression system for producing a protein in a filamentous fungus, consisting of a) a host organism selected from the species Trametes and Polyporus and b) a DNS vector containing a selection marker gene, said selection marker gene coding a protein which allows the selection of positive transformants after the transformation of the host organism and which is selected from the group of: antibiotics-resistant genes coding for proteins which eliminate the growth-inhibitory effect of antibiotics against which the host organism is not resistant; genes coding proteins which are capable of a colour-causing reaction; and genes complementing a genetic defect of the host organism (auxotrophy), the expression of the selection marker gene being controlled by at least one active genetic regulation element in the host organism, and c) a DNS vector containing a gene which codes the protein to be produced, the expression of this gene and optionally, the secretion of the protein so produced being controlled by an active genetic regulation element in the host organism. The DNS vector containing a section marker gene and the DNS vector containing the gene which codes the protein to be produced can also be in the form of one DNS vector.