发明名称 |
Method of enriching rare cells |
摘要 |
A method for enriching cancer cells such as prostate cancer cells in a bodily fluid sample is disclosed comprising (a) obtaining the sample comprising cancer cells and non-rare cells; (b) subjecting the sample to multiple density gradient separation comprising a first density gradient and a second density gradient, wherein the second density gradient is greater than the first density gradient, and producing a first fluid comprising an increased concentration of cancer cells of a first density, and a second fluid comprising an increased concentration of cancer cells of a second density, wherein the second density is greater than the first density; wherein subjecting the sample to multiple density gradient separation includes producing a plasma layer, a first interface layer, a first gradient layer, a second interface layer, a second gradient layer, and a cell pellet; wherein producing the first fluid includes combining the first interface layer and the first gradient layer and forming a first suspension; and wherein producing the second fluid includes combining the second interface layer and the second gradient layer and forming a second suspension; (c) subjecting said second fluid comprising the second suspension to a binding agent that binds non-rare cells; and (d) removing the bound non-rare cells from the second fluid to provide a second fluid enriched with the greater density cancer cells.
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申请公布号 |
US5962237(A) |
申请公布日期 |
1999.10.05 |
申请号 |
US19970832468 |
申请日期 |
1997.04.02 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE |
发明人 |
TS'O, PAUL O. P.;WANG, ZHENG-PIN;LESKO, STEPHEN A.;NELSON, WILLIAM G.;PARTIN, ALAN W. |
分类号 |
C12N15/09;C12N5/06;C12N5/08;C12N5/10;C12Q1/68;G01N33/48;G01N33/569;G01N33/574;(IPC1-7):G01N33/574;G01N33/53;G01N9/30;B01D33/15 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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