Process for producing D-amino acids with composite immobilized enzyme preparation

摘要

PCT No. PCT/JP95/01257 Sec. 371 Date Aug. 16, 1996 Sec. 102(e) Date Aug. 16, 1996 PCT Filed Jun. 23, 1995 PCT Pub. No. WO96/00296 PCT Pub. Date Jan. 4, 1996A process for the efficient production of a D-amino acid from the corresponding DL-5-substituted hydantoin by one-step reaction which comprises using a composite immobilized enzyme at a pH about neutrality, said composite immobilized enzyme being obtained by immobilizing a hydantoinase having its optimal pH within an alkaline range and a D-N-carbamyl- alpha -amino acid amidohydrolase having its optimal pH about neutrality in a coexisting state on an immobilizing support, simultaneously, is disclosed.