| 摘要 |
<p>A method for assaying UTI which comprises inactivating α1-antitrypsin (α1-AT) in a specimen, mixing a trypsin solution with the specimen, then adding a substrate to thereby initiate an enzyme reaction, and determining UTI by measuring absorbance, etc. The α1-AT may be inactivated by adding a protease other than trypsin to the specimen and reacting the protease with α1-AT to thereby form a complex, or by adding an oxidizing agent to the specimen. As the protease described above, use may be made of elastase or subtilisin. As the oxidizing agent described above, use may be made of sodium iodate, iodine, copper sulfate and iron trichloride. As the graph in the figure shows, α1-AT in the specimen is inactivated by adding subtilisin and thus the amount of UTI can be accurately determined.</p> |
