| 摘要 |
<p>PROBLEM TO BE SOLVED: To provide the subject new protein and relating polypeptide composed of essentially pure alveolus surface-active protein human SP18 monomer protein and useful e.g. for the production of a synthetic alveolus surface-active protein for the treatment of respiratory distress syndrome of the newborn, etc. SOLUTION: This protein is an essentially pure new alveolus surface-active protein human SP18 monomer protein useful e.g. as an active component of a composition containing a synthetic alveolus surface-active protein for the effective surface-active substance substitution treatment of respiratory distress syndrome(RDS) of the newborn, etc., caused by the deficiency of alveolus surface active substance. The surface-active protein can be produced by separating a human alveolus surface active substance from whole-phase semimembranous liquid, subjecting to anion exchange column treatment in a tris-EDTA buffer containing 1% n-octyl-β-D-glucopyranoside by conventional method, extracting the fraction passed through the column with 2:1 chloroform : methanol, distilling out the solvent from the extract and fractionating the extracted component by high-speed liquid chromatography.</p> |
