A method of producing cloned nuclear transfer embryos from differentiated donor cells is described. The method includes culturing differentiated donor cells in low serum medium and using bovine oocytes as recipient oocytes for donor cells from different species. This method may be used to produce genetically identical animals and transgenic animals.
申请公布号
WO9905266(B1)
申请公布日期
1999.09.23
申请号
WO1998US15387
申请日期
1998.07.24
申请人
WISCONSIN ALUMNI RESEARCH FOUNDATION;FIRST, NEAL, L.;DOMINKO, TANJA;MITALIPOUA, MAISSAM