A SIMPLIFIED SYSTEM FOR GENERATING RECOMBINANT ADENOVIRUSES
摘要
Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. This invention describes a strategy which simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, employing homologous recombination in bacteria rather than in eucaryotic cells. Following transfections of such plasmids into a mammalian packaging cell line, viral production can be conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system expedites the process of generating and testing recombinant adenoviruses.
申请公布号
WO9943843(A1)
申请公布日期
1999.09.02
申请号
WO1999US04062
申请日期
1999.02.25
申请人
JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE;HE, TONG-CHUAN;VOGELSTEIN, BERT;KINZLER, KENNETH, W.
发明人
HE, TONG-CHUAN;VOGELSTEIN, BERT;KINZLER, KENNETH, W.