TRANSMEMBRANE TRAPPING METHODS

摘要

The present invention provides a method of cloning a protein coding for a membrane protein without the need of using any antibody or ligand. The method of cloning a membrane protein comprises selecting a protein having a signal sequence, using a DNA coding for the signal sequence and N-terminal sequence of the protein as the reporter gene, and screening for a gene for a protein having a transmembrane domain(s). The present invention is concerned also with the cDNA obtained by using such method, a vector containing the DNA, a transformant as transformed with the vector, and the transcription product of the DNA as produced by the transformant. <IMAGE>