METHOD AND APPARATUS FOR INTRODUCING SUBSTANCES INTO THE CELL PLASMA MEMBRANE AND/OR CYTOSOL
摘要
There is disclosed a method for introducing agents into the cytosol and/or plasma membrane of a cell. The method comprises the steps of: (a) coating at least a portion of a tip of a transferring means with a lipid; (b) bringing the lipid-coated tip of the transferring means into contact with the cell; and (c) transferring the contents of the transferring means into the cytosol and/or plasma membrane of the cell without entering the cytoplasm. Also disclosed is a transferring means for injecting agents into the cytosol and/or plasma membrane of the cell and a method of coating a tip of a transferring means with a lipid. A suitable packaging and a method for preparing the transferring means for use are also disclosed.
申请公布号
WO9941401(A1)
申请公布日期
1999.08.19
申请号
WO1999GB00475
申请日期
1999.02.16
申请人
UNIVERSITY OF WALES COLLEGE OF MEDICINE;LAFFAFIAN, IRAJ;HALLETT, MAURICE, BARTLETT