CONTAINED SAMPLING DEVICE

摘要

The contained sampling device of the present invention for taking sterile samples of a fluid such as biological samples from a fermenter or bio-reactor provides a simplified and completely contained system for collecting sterile samples. The contained sampling device includes a device body having a first fluid connection for connecting to a source of material to be sampled and a second fluid connection for connecting to a waste container. A bottle seat is provided on the device body for receiving a sampling bottle neck in fluid communication with the device body. First and second fluid paths extend through the device body for delivering fluid from the source of material to the sampling body or the waste container. Another embodiment of a sampling apparatus includes a sample collecting bottle holder in fluid communication with the fermenter or bio-reactor for removably connecting sample collecting containers. An adaptor is removably connected to the holder for steam sterilization of the holder between collection of successive samples. These systems for sterile sampling prevent contamination of the biological samples and also prevents exposure of workers to the sample media by allowing the purging of the sampling device after sample collection.