| 摘要 |
<p>A method and apparatus for performing determinations of allergen-specific IgE antibodies in biological fluids includes a kit with coated, color coded test units for insertion into respective reaction containers containing the test fluid. If there is an antibody specific to the allergen coated on any of the test unit tips, that antibody becomes bound to the associated allergen in the corresponding reaction container. The tips are rinsed and inserted into a second set of reaction containers with an enzyme labeled antibody conjugate. The test units are removed, rinsed and placed in a third set of reaction containers containing chromogenic substrate of hydrogen peroxide and TMB (tetra methyl benzidine). Upon positive reaction with a tip, the chromogenic substrate develops a specific color. In one embodiment, a sealed reagent kit is provided having predispensed, color coded, liquid reagents including an incubation medium of a buffered protein solution containing preservatives; a second liquid reagent is an antibody conjugate in a buffer with stabilizers and preservatives; a third liquid reagent is a chromogenic substrate, a stabilized and buffered solution of peroxide and TMB. The kit includes calibrator solutions and a plurality of tips.</p> |
