METHOD FOR CLONING SECRETED PROTEINS

摘要

The invention provides a method for isolating nucleic acids encoding proteins comprising a signal peptide, e.g. a secreted protein. The method of the invention comprises isolating an RNA molecule associated with an endoplasmic reticulum membrane preparation under conditions wherein the RNA is at least partially translated. The invention also provides a library of nucleic acids encoding proteins comprising a signal peptide, individual nucleic acids isolated according to the method of invention, peptides encoded thereby, pharmaceutical compositions comprising such and kits for performing the method of the invention.