发明名称 NOVEL POTASSIUM CHANNEL PROTEIN
摘要 A novel potassium channel protein expressed exclusively in the brain: a gene encoding this protein; a vector containing this gene; a host cell containing this vector; etc. A typical method for obtaining the above protein is as follows: mRNA is extracted from human cells or tissue capable of producing the target potassium channel protein. Then use is made of two primers between which the channel protein mRNA or a part of the mRNA region is located by using the thus extracted mRNA as a template. By effecting a reverse transcriptase-polymerase chain reaction (RT-PCR), the channel protein cDNA or a part thereof can be obtained. The obtained novel potassium channel cDNA or a part of the same is integrated into an appropriate expression vector and then expressed in a host cell. The target protein can be thus produced.
申请公布号 WO9937677(A1) 申请公布日期 1999.07.29
申请号 WO1999JP00190 申请日期 1999.01.20
申请人 YAMANOUCHI PHARMACEUTICAL CO., LTD.;MIYAKE, AKIRA;MOCHIZUKI, SHINOBU;YOKOI, HIROMICHI 发明人 MIYAKE, AKIRA;MOCHIZUKI, SHINOBU;YOKOI, HIROMICHI
分类号 C07K14/705;C12N1/21;(IPC1-7):C07K14/47;C12N15/10;C12N5/10;C12P21/02 主分类号 C07K14/705
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