摘要 |
<p>PROBLEM TO BE SOLVED: To provide a method for culturing a virus or a cell by effectively supplying oxygen to a culture solution in a method for culturing a virus or a cell. SOLUTION: In this method for culturing a virus or a cell in a suspension state, a sufficient amount of oxygen is supplied to a culture solution without directly bringing an oxygen gas into contact with a virus. In a method for culturing a virus and a cell in a suspension state or a method for culturing a cell in a suspension state, an oxygen supplying method comprising (a) a process for supplying a hydrophobic solvent having dissolved oxygen to a culture tank, (b) a process for taking out the hydrophobic solvent from the culture tank to the outside of the tank, (c) a process for dissolving oxygen in the hydrophobic solvent taken out from the culture tank to the outside of the tank by using an oxygen permeable membrane and (d) a process for recycling the obtained hydrophobic solvent having dissolved oxygen to the process (a) is used.</p> |