发明名称 |
Mass spectrometric methods for sequencing nucleic acids |
摘要 |
A mass spectrometric method for sequencing nucleic acids using RNA polymerases, including DNA-dependent and RNA-dependent RNA polymerases, is provided. The methods use a modified Sanger sequencing strategy in which RNA polymerase is used to generate a set of nested RNA transcripts obtained by base-specific chain termination. These are analyzed by mass spectrometry. A method of identifying transcriptional terminator sequences or attenuator sequences is also provided. |
申请公布号 |
AU1918799(A) |
申请公布日期 |
1999.07.05 |
申请号 |
AU19990019187 |
申请日期 |
1998.12.15 |
申请人 |
SEQUENOM, INC.;KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY;MARYANNE J. O'DONNELL |
发明人 |
MARYANNE J. O'DONNELL;CHANGWON KANG;YOUNG-SOO KWON;YOUNG TAE KIM;HUBERT KOSTER;DANIEL P. LITTLE;GUOBING XIANG;DAVID M. LOUGH;CHARLES CANTOR |
分类号 |
C12N15/09;C12Q1/68;C40B40/06;C40B60/14 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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