| 摘要 |
PCT No. PCT/SE94/01088 Sec. 371 Date Apr. 25, 1996 Sec. 102(e) Date Apr. 25, 1996 PCT Filed Nov. 17, 1994 PCT Pub. No. WO95/14035 PCT Pub. Date May 26, 1995The invention relates to a method for purification of a mixture of hydroxamate derivatized protein/proteins and native protein which is characterized by treating the mixture with immobilized metal and thereby adsorbing the hydroxamate derivatized protein/proteins on the immobilized-metal and recovering the native protein. The protein could be IGF-L. It also relates to a process for the production of a native protein which is characterized by expression of the protein as a fusion protein, cleavage of the fusion protein by hydroxylanine, separation of native protein from hydroxamate derivatized protein by adsorbing the hydroxamate derivatized protein on immobilized metal and directly recovering the native protein. The use of immobilized metal affinity chromatography for separation of native protein from hydroxamate derivatized protein is also claimed.
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