LACTOSE REPRESSOR PROTEINS WITH ALTERED LIGAND RESPONSIVITY

摘要

The present invention provides altered lac repressor proteins that recognize the lactose operator but have an altered ligand responsivity. The altered ligand responsivity provides that a sugar or other small molecule other than allolactose or IPTG acts as an inducer for the altered lac repressor protein or that IPTG acts at lower concentrations. DNA sequences encoding the altered lac repressor proteins and bacterial and eukaryotic cells containing altered lac repressor proteins are also provided.