发明名称 |
Process to prepare proteins by controlled proteolysis of pro-proteins |
摘要 |
Production of proteins from pro-proteins comprises contacting a pro-protein solution with a protease and a solid support that has a higher affinity for the proteins than for the pro-protein or its functionally inactive degradation products, whereby the pro-protein is proteolytically cleaved to form the protein, which is selectively separated by adsorption on the solid support. Also claimed are: (1) a hirudin or a hirudin fragment or derivative coupled to a solid support; (2) a protein complex produced by the above process, comprising a protein adsorbed on a solid carrier, and (3) an apparatus for carrying out the above process, comprising a container containing a protease directly connected to a container containing a solid support. |
申请公布号 |
EP0776969(A3) |
申请公布日期 |
1999.06.02 |
申请号 |
EP19960890172 |
申请日期 |
1996.11.19 |
申请人 |
IMMUNO AKTIENGESELLSCHAFT |
发明人 |
FISCHER, BERNHARD, DOZ.;MITTERER, ARTUR, DR.;DORNER, FRIEDRICH, PROF.;EIBL, JOHANN, DR. |
分类号 |
C12P21/00;A61K38/43;A61K38/46;A61K38/55;A61P7/00;A61P7/02;C07K1/04;C07K1/22;C07K14/745;C07K19/00;C12N9/50;C12N9/64;C12N9/74;C12P21/06 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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