| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject new translational control protein comprising a protein having a specific molecular weight and capable of being bound to a sequence controlling the transcription of cathepsin B protein, capable of controlling the transcription of the mRNA of the cathepsin B protein, and useful for elucidation, etc., of the mechanism of the transcription control. SOLUTION: This translational control protein is the new one having about 90 kDa molecular weight and capable of being bound to a sequence controlling the transcription of a cathepsin B protein, controls the transcription of mRNA of cathepsin B, etc., and is useful for elucidation, etc. of the mechanism of the transcription control. The protein can be obtained by homogenating cultured cells derived from an embryo of Sarcophaga peregrina, removing nucleuses from the homogenated cultured cells by a centrifugal separation to provide a supernatant fraction, adding ammonium sulfate with 50% final concentration to the obtained supernatant, removing the precipitate from the supernatant with the added ammonium sulfate, further adding the ammonium sulfate with 80% final concentration to the resultant supernatant to recover the insolubilized protein, and fractionating the insolubilized protein by using a column chromatography and RNA-binding activities. The amount of the transcription by a mRNA with deleted 3'-nontranscription region is recognized larger than that by the whole length mRNA by the test of the control of a relative amount of the protein transcription at the RNA-binding site of the protein by various RNAs. |
