| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new protein comprising an outer membrane protein having a specific amino acid sequence and a prescribed molecular weight, related to an epitome of Haemophilus influenza called PBOMP-2, useful for an influenza vaccine, etc. SOLUTION: This new substantially pure antigenic peptide or protein comprises an outer membrane protein having an amino acid sequence composed of an amino acid sequence of the formula and about 16,000 dalton molecular weight, is related to an epitome of Haemophilus influenza called PBOMP-2 and is useful as an immunogen in a vaccine formulation for generating an antibody useful in active immunization and passive immunization against influenza bacillus, a diagnostic, etc. The protein is obtained by isolating an insoluble cell wall fraction rich in an outer membrane protein from a physically destroyed cell of Haemophilus influenza, heating the fraction in the presence of a surfactant or digesting the fraction with a lysozyme. |
