发明名称 |
Vorrichtung und Verfahren zum schnellen und hochempfindlichen Erkennen und Zählen von Mikroorganismen mittels Fluoreszenz |
摘要 |
Apparatus for rapid, ultrasensitive and automatic counting of fluorescent biological cells such as microorganims, carried by a solid support such as a filter and process for said rapid count of said microorganisms by said apparatus. Said process comprises: scanning a solid support on which a sample potentially containing microorganisms has been deposited, with an incident beam from a laser, forming a laser spot on the solid support, said laser spot being substantially greater than the microorganisms to be detected, said laser spot size being comprised between 4 and 14 mu m and simultaneously: detecting the resultant fluorescent light at least at one wavelength; establishing a set of correlated-features by a line-to-line correlation of individual features; comparing said correlated-features on each pair of adjacent lines in time synchrony, at least at two different wavelengths lambda 1 and lambda 2; making a size discrimination of retained events and selecting those having a size corresponding to a microorganism; determining if for retained events after size discrimination, the events energy profile in three dimensions is within predetermined Gaussian shape criteria; and counting said remaining events to determine and to count exclusively the microorganisms present on said solid support. <IMAGE> |
申请公布号 |
DE69417900(D1) |
申请公布日期 |
1999.05.20 |
申请号 |
DE1994617900 |
申请日期 |
1994.11.17 |
申请人 |
CHEMUNEX, MAISONS-ALFORT, FR |
发明人 |
DROCOURT, JEAN-LOUIS, F-91330 YERRES, FR;DESFETES, PATRICK, F-95350 SAINT BRICE SOUS FORET, FR;SANGHERA, JASPAL, FOXTON, CAMBRIDGE CB2 6SP, GB |
分类号 |
C12M1/34;C12Q1/06;G01N15/14;(IPC1-7):G01N15/14 |
主分类号 |
C12M1/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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