摘要 |
PCT No. PCT/EP96/00186 Sec. 371 Date Feb. 17, 1998 Sec. 102(e) Date Feb. 17, 1998 PCT Filed Jan. 17, 1996 PCT Pub. No. WO96/41163 PCT Pub. Date Dec. 19, 1996The invention concerns a method for determining the action of effectors on target cells, wherein thawed cryopreserved mammalian cells are used without prior culture as target cells which have a vitality of at least 85% after storage at -70 DEG C. for a period of 4 weeks and are obtainable by preparing a suspension of the target cells in a medium, adding a cryopreservative up to a final concentration of 2-10% (v/v) and freezing the cells with a temperature decrease of no more than 2 DEG C. per minute.
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