| 摘要 |
A method for efficiently screening full-length cDNA clones which comprises: determining the base sequence in the 5'-region of each clone contained in a cDNA library prepared by a method for constructing a cDNA library involving full-length ones at a high ratio; examining the presence/absence of initiation ATG in this 5'-region and the location thereof by using an originally developed software for anticipating initiation codons in cDNA; thus exactly judging the presence/absence of the initiation codon and the location thereof; and screening the cDNAs thus judged as carrying the initiation codon from the cDNA library. Moreover, a cDNA library containing full-length ones at an extremely high ratio can be constructed by mixing the clones thus selected above. |
