发明名称 |
EVALUATION OF CHANGES IN BREAST CELLS USING NIPPLE ASPIRATE FLUID |
摘要 |
This invention provides a method for studying chromosomal gains and losses in cells from nipple aspirate fluid comprising the steps of: (1) aspirating fluid from breasts; (2) placing the samples of fluid obtained in step 1 onto dishes containing conditioned medium composed of a mixture of (a) supernatant from immortalized mammary epithelial cells and (b) mammary epithelial growth medium; (3) incubating the product of step 2 in a humidified incubator; (4) replenishing the medium in the dishes prepared in step 2 at regular intervals to maintain cell growth; (5) isolating the cells from the cultures; (6) preparing DNA from these cells; (7) amplifying the DNA; (8) labelling an aliquot of the amplified DNA; and (9) evaluating the labelled DNA for evidence of chromosomal gains or losses.
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申请公布号 |
WO9921964(A1) |
申请公布日期 |
1999.05.06 |
申请号 |
WO1998US22478 |
申请日期 |
1998.10.23 |
申请人 |
GEORGETOWN UNIVERSITY |
发明人 |
DICKSON, ROBERT, B.;HADDAD, BASSEM, R.;MCCORMACK, STEPHEN, J. |
分类号 |
C12N5/08;C12N15/12;C12Q1/68;(IPC1-7):C12N5/00;C12P19/34;C12Q1/02;C12Q1/04 |
主分类号 |
C12N5/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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