ZOOGLOEAL AND HYPHOMICROBIUM SPP. NUCLEIC ACIDS

摘要

The invention provides two new zoogloeal strains, mzlt and mz2t. The invention provides an isolated nucleic acid consisting of the nucleic acid of SEQ ID NO: 1. Examples of nucleic acids of mz1t include SEQ ID NO: 2 and SEQ ID NO: 3. An example of a nucleic acid of mz2t is SEQ ID NO: 4. The invention also provides an isolated nucleic acid consisting of the nucleic acid of SEQ ID NO: 5. A method of detecting the presence of zoogloeal clusters in a wastewater sample is provided, comprising: a) contacting RNA from a sample of the wastewater with a nucleic acid comprising the nucleic acid of SEQ ID Nos: 1, 2, 3, 4 or 5 under conditions that permit specific hybridization; and b) detecting the presence of hybridization, the presence of hybridization indicating the presence of zoogloeal clusters. A new Hyphomicrobium spp. strain, designated M3, is provided herein. The invention provides novel nucleic acids of a Hyphomicrobium sp. M3. For example SEQ ID NO: 6 and SEQ ID NO: 7 rDNAs of M3. SEQ ID Nos: 8, 9, 10, 11, 12, 13 and 14 are presumptive Hyphomicrobium spp. sequences. SEQ ID NO: 15 is a consensus sequence from 6m3 and 3m3 (Hyphomicrobium sp. m3 isolated from sludge). A method of detecting the presence of a Hyphomicrobium species in a wastewater sample as described above, using a nucleic acid comprising the nucleic acid of SEQ ID NO: 17 is also provided. The nucleic acids of SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15 can also be utilized in whole or as fragments in the detection method for Hyphomicrobium spp.