发明名称 |
METHOD TO SCREEN PHAGE DISPLAY LIBRARIES WITH DIFFERENT LIGANDS |
摘要 |
The invention provides a method for selecting, from a repertoire of polypeptides, a population of functional polypeptides which bind a target ligand in a first binding site and a generic ligand in a second binding site, which generic ligand is capable of binding functional members of the repertoire regardless of target ligand specificity, comprising the steps of: a) contacting the repertoire with the generic ligand and selecting functional polypeptides bound thereto; and b) contacting the selected functional polypeptides with the target ligand and selecting a population of polypeptides which bind to the target ligand. The invention accordingly provides a method by which a polypeptide repertoire is preselected, according to functionality as determined by the ability to bind the generic ligand, and the subset of polypeptides obtained as a result of such preselection is then employed for further selection according to the ability to bind the target ligand. |
申请公布号 |
WO9920749(A1) |
申请公布日期 |
1999.04.29 |
申请号 |
WO1998GB03135 |
申请日期 |
1998.10.20 |
申请人 |
MEDICAL RESEARCH COUNCIL;TOMLINSON, IAN;WINTER, GREG |
发明人 |
TOMLINSON, IAN;WINTER, GREG |
分类号 |
G01N33/50;C07K1/04;C07K14/705;C07K14/725;C07K16/00;C12N15/09;C12N15/10;C12P21/08;C40B30/04;C40B40/02;G01N33/15;G01N33/68 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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